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Image Search Results
Journal: Pathology and Oncology Research
Article Title: Knockdown of GPSM1 Inhibits the Proliferation and Promotes the Apoptosis of B-Cell Acute Lymphoblastic Leukemia Cells by Suppressing the ADCY6-RAPGEF3-JNK Signaling Pathway
doi: 10.3389/pore.2021.643376
Figure Lengend Snippet: Downregulation of GPSM1 suppressed cell proliferation, induced cell cycle arrest and promoted apoptosis in BALL-1 and Reh cells. (A) Expression of GPSM1 mRNA in the human B lymphoblast cell line HMy2.CIR and several human leukemia cell lines (BALL-1, Jurkat and Reh). GAPDH was used as an internal reference for normalization. * p < 0.05 vs. the HMy2.CIR group. (B) GPSM1 protein expression in the human B lymphoblast cell lineHMy2.CIR and several human leukemia cell lines (BALL-1, Jurkat and Reh) was investigated by western blotting. (C) The grayscale value of the GPSM1 protein band was quantified by Tanon Image software, and GAPDH was used as an internal reference. * p < 0.05 vs. the HMy2.CIR group. (D) Relative mRNA expression in sh-Con- and sh-GPSM1-transfected BALL-1 and Reh cells. (E) GPSM1 protein expression in sh-Con- and sh-GPSM1-transfected BALL-1 and Reh cells. (F) The grayscale value of the GPSM1 protein band was quantified by Tanon Image software, and GAPDH was used as an internal reference. * p < 0.05, compared with the sh-Con group. (G) The CCK-8 assay was used to evaluate the cell proliferation ability of BALL-1 and Reh cells transfected with sh-GPSM1 and sh-Con on four consecutive days. * p < 0.05, ** p < 0.01, compared with the sh-Con group. (H,I) The cell cycle was examined by PI staining using flow cytometry. (J) Cell apoptosis rates were assessed by flow cytometry after staining cells with annexin V-PE/7-AAD. Nonapoptotic cells: annexin V-PE − /7-AAD − , early apoptotic cells: annexin V-PE + /7-AAD − , late apoptotic cells: annexin V-PE + /7-AAD + . PE, phycoerythrin; 7-AAD, 7-amino-actinomycin D. (K) The total apoptosis rate is the sum of the early apoptosis rate and the late apoptosis rate. * p < 0.05, compared with the sh-Con group. All data are expressed as the mean ± SD.
Article Snippet: A
Techniques: Expressing, Western Blot, Software, Transfection, CCK-8 Assay, Staining, Flow Cytometry